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唾液酸结合Ig样凝集素8(SIGLEC8)重组蛋白

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产品名称: 唾液酸结合Ig样凝集素8(SIGLEC8)重组蛋白
产品型号: SIGLEC8
产品展商: 蛋白/多肽/抗原
产品文档: 无相关文档

简单介绍

唾液酸结合Ig样凝集素8(SIGLEC8)重组蛋白蛋白质的不同在于其氨基酸的种类、数目、排列顺序和肽链空间结构的不同,蛋白质的氨基酸序列由对应基因所编码,空间结构决定于氨基酸序列本身,通过氢键或范德华力等作用力实现。蛋白质不仅具有上等结构,还具有二级、三级结构,甚至四级结构。上等结构只是蛋白质的基本结构,称之为低级结构。唾液酸结合Ig样凝集素8(SIGLEC8)重组蛋白如果想让蛋白质有更多的生物功能则必须赋予它们更复杂的空间结构,称之为上等结构。应用重组DNA将目标基因进行表达得到的


唾液酸结合Ig样凝集素8(SIGLEC8)重组蛋白  的详细介绍

唾液酸结合Ig样凝集素8(SIGLEC8)重组蛋白

  • 物种Homo sapiens (Human,人)相同的名称,不同的物种。
  • 预测分子量59.0kDa来源原核表达
  • 宿主E.coli
  • 内**水平<1.0EU/µg(LAL法测定)
  • 亚细胞定位n/a
  • 实际分子量-(差异分析请参阅说明书)
  • 片段与标签Gly70~Ser336 with N-terminal His Tag
  • 缓冲液成份磷酸盐缓冲液(pH7.4,含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.)
  • 性状冻干粉
  • 纯度> 95%
  • 等电点-
  • 唾液酸结合Ig样凝集素8(SIGLEC8)重组蛋白应用SDS-PAGE; WB; ELISA; IP
    用法
    PBS或其它。
    储存
    避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。
    稳定性
    热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。
    唾液酸结合Ig样凝集素8(SIGLEC8)重组蛋白


    Recombinant Sialic Acid Binding Ig Like Lectin 8 (SIGLEC8)

    Organism Species: Homo sapiens (Human)

    Instruction manual

    FOR IN VITRO USE AND RESEARCH USE ONLY

    NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

    10th Edition (Revised in Jan, 2014)

    唾液酸结合Ig样凝集素8(SIGLEC8)重组蛋白[ PROPERTIES ]

    Residues: Gly70~Ser336

    Tags: Two N-terminal Tags, His-tag and GST-tag

    Accession: Q9NYZ4

    Host: E. coli

    Subcellular Location: Membrane; Single-pass type

    I membrane protein.

    Purity: >95%

    Endotoxin Level: <1.0EU per 1μg

    (determined by the LAL method).

    Formulation: Supplied as lyophilized form in 20mM Tris,

    150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,

    0.01% sarcosyl, 5% trehalose, and preservative.

    Predicted isoelectric point: 9.2

    Predicted Molecular Mass: 59.0kDa

    Applications: SDS-PAGE; WB; ELISA; IP.

    (May be suitable for use in other assays to be determined by the end user.)

    [ USAGE ]

    Reconstitute in sterile ddH2O.

    [ STORAGE AND STABILITY ]

    Storage: Avoid repeated freeze/thaw cycles.

    Store at 2-8

    oC for one month.

    Aliquot and store at -80oC for 12 months.

    Stability Test: The thermal stability is described by the loss rate of the target

    protein. The loss rate was determined by accelerated thermal degradation test,

    that is, incubate the protein at 37oC for 48h, and no obvious degradation and

    precipitation were observed. (Referring from China Biological Products Standard,

    which was calculated by the Arrhenius equation.) The loss of this protein is less

    than 5% within the expiration date under appropriate storage condition.

    唾液酸结合Ig样凝集素8(SIGLEC8)重组蛋白[ SEQUENCES ]

    The sequence of the target protein is listed below.

    G DRPYQDAPVA TNNPDREVQA ETQGRFQLLG DIWSNDCSLS IRDARKRDKG

    SYFFRLERGS MKWSYKSQLN YKTKQLSVFV TALTHRPDIL ILGTLESGHS RNLTCSVPWA

    CKQGTPPMIS WIGASVSSPG PTTARSSVLT LTPKPQDHGT SLTCQVTLPG TGVTTTSTVR

    LDVSYPPWNL TMTVFQGDAT ASTALGNGSS LSVLEGQSLR LVCAVNSNPP ARLSWTRGSL

    TLCPSRSSNP GLLELPRVHV RDEGEFTCRA QNAQGS

    [ REFERENCES ]

    1. Varela I., et al. (2011) Nature 469:539-542.

    2. Na H.J., et al. (2012) J. Immunol. Methods 383:39-46.

    3. Na H.J., et al. (2012) Cytokine 57:169-174.

    4. Hudson S.A., et al. (2011) J. Clin. Immunol. 31:1045-1053.


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