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线粒体超氧化物歧化酶(SOD2)重组蛋白

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产品名称: 线粒体超氧化物歧化酶(SOD2)重组蛋白
产品型号: SOD2
产品展商: 蛋白/多肽/抗原
产品文档: 无相关文档

简单介绍

线粒体超氧化物歧化酶(SOD2)重组蛋白蛋白质的不同在于其氨基酸的种类、数目、排列顺序和肽链空间结构的不同,蛋白质的氨基酸序列由对应基因所编码,空间结构决定于氨基酸序列本身,通过氢键或范德华力等作用力实现。蛋白质不仅具有上等结构,还具有二级、三级结构,甚至四级结构。上等结构只是蛋白质的基本结构,称之为低级结构。线粒体超氧化物歧化酶(SOD2)重组蛋白如果想让蛋白质有更多的生物功能则必须赋予它们更复杂的空间结构,称之为上等结构。应用重组DNA将目标基因进行表达得到的


线粒体超氧化物歧化酶(SOD2)重组蛋白  的详细介绍

线粒体超氧化物歧化酶(SOD2)重组蛋白

  • 物种Rattus norvegicus (Rat,大鼠)相同的名称,不同的物种。
  • 性状冻干粉内**水平<1.0EU/µg(LAL法测定)
  • 亚细胞定位n/a
  • 预测分子量23.5kDa
  • 实际分子量-(差异分析请参阅说明书)
  • 片段与标签Lys25~Lys222 (Accession # P07895) with N-terminal His Tag
  • 缓冲液成份磷酸盐缓冲液(pH7.4,含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.)
  • 纯度> 95%
  • 等电点-
  • 应用SDS-PAGE; WB; ELISA; IP
    线粒体超氧化物歧化酶(SOD2)重组蛋白用法
    PBS或其它。
    储存
    避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。
    稳定性
    热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。
    线粒体超氧化物歧化酶(SOD2)重组蛋白


    Superoxide Superoxide Superoxide Superoxide Dismutase Dismutase Dismutase Dismutase 2, Mitochondrial Mitochondrial Mitochondrial Mitochondrial (SOD2)

    Organism: Organism: Organism: Organism: Rattus norvegicus norvegicus norvegicus norvegicus (Rat)

    Instruction Instruction Instruction Instruction manual

    FOR IN VITRO USE AND RESEARCH USE ONLY

    NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES

    4th Edition (Revised in August, 2012)

    线粒体超氧化物歧化酶(SOD2)重组蛋白[ DESCRIPTION DESCRIPTION DESCRIPTION DESCRIPTION ]

    Protein Protein Protein Protein Names: Superoxide Dismutase 2, Mitochondrial

    Synonyms Synonyms Synonyms Synonyms: SOD2

    Species: Species: Species: Species: Rat

    Size: 100µg

    Source: Escherichia coli-derived

    Subcellular Subcellular Subcellular Subcellular Location: Location: Location: Location: Mitochondrion matrix.

    [ PROPERTIES ROPERTIES ROPERTIES ROPERTIES ]

    Residues Residues Residues Residues: Lys25~Lys222 (Accession # P07895), with N-terminal His-Tag.

    Grade & Purity: Purity: Purity: Purity: >95%, 24 kDa as determined by SDS-PAGE reducing conditions.

    Formulation Formulation Formulation Formulation: Supplied as liquid form in Phosphate buffered saline(PBS), pH 7.4.

    Endotoxin Endotoxin Endotoxin Endotoxin Level: <1.0 EU per 1μg (determined by the LAL method). Applications: Applications: Applications: Applications: SDS-PAGE; WB; ELISA; IP.

    (May be suitable for use in other assays to be determined by the end user.)

    Predicted Predicted Predicted Predicted Molecular Molecular Molecular Molecular Mass: 23.5 kDa

    Predicted Predicted Predicted Predicted isoelectric isoelectric isoelectric isoelectric point: 7.9

    [ PREPARATION PREPARATION PREPARATION PREPARATION ]

    Reconstitute in sterile PBS, pH7.2-pH7.4.

    [ STORAGE STORAGE STORAGE STORAGE AND STABILITY STABILITY STABILITY STABILITY ]

    Storage: Storage: Storage: Storage: Avoid repeated repeated repeated repeated freeze/thaw freeze/thaw freeze/thaw freeze/thaw cycles. cycles. cycles. cycles.

    Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.

    Stability Stability Stability Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was

    determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious

    degradation and precipitation were observed. (Referring from China Biological Products Standard, which was

    calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under

    appropriate storage condition.

    线粒体超氧化物歧化酶(SOD2)重组蛋白[ SEQUENCES SEQUENCES SEQUENCES SEQUENCES ]

    The target protein is fused with N-terminal His-tag, its sequence is listed below.

    MGHHHHHHSGS-KHSLPD LPYDYGALEP HINAQIMQLH HSKHHATYVN NLNVTEEKYH EALAKGDVTT QVALQPALKF

    NGGGHINHSI FWTNLSPKGG GEPKGELLEA IKRDFGSFEK FKEKLTAVSV GVQGSGWGWL GFNKEQGRLQ IAACSNQDPL

    QGTTGLIPLL GIDVWEHAYY LQYKNVRPDY LKAIWNVINW ENVSQRYIVC KK


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