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血管生长素(ANG)重组蛋白

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产品名称: 血管生长素(ANG)重组蛋白
产品型号: ANG
产品展商: 蛋白/多肽/抗原
产品文档: 无相关文档

简单介绍

血管生长素(ANG)重组蛋白蛋白质的不同在于其氨基酸的种类、数目、排列顺序和肽链空间结构的不同,蛋白质的氨基酸序列由对应基因所编码,空间结构决定于氨基酸序列本身,通过氢键或范德华力等作用力实现。蛋白质不仅具有上等结构,还具有二级、三级结构,甚至四级结构。上等结构只是蛋白质的基本结构,称之为低级结构。血管生长素(ANG)重组蛋白如果想让蛋白质有更多的生物功能则必须赋予它们更复杂的空间结构,称之为上等结构。应用重组DNA将目标基因进行表达得到的


血管生长素(ANG)重组蛋白  的详细介绍

血管生长素(ANG)重组蛋白


  • 物种Bos taurus; Bovine (Cattle,牛)相同的名称,不同的物种。
  • 片段与标签Ala24~His148 with N-terminal His Tag宿主E.coli
  • 内**水平<1.0EU/µg(LAL法测定)
  • 亚细胞定位分泌
  • 预测分子量15.9kDa
  • 实际分子量18kDa(差异分析请参阅说明书)
  • 缓冲液成份20mM Tris, 150mM NaCl缓冲液(pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300)
  • 性状冻干粉
  • 纯度> 97%
  • 等电点9.2
  • 应用SDS-PAGE; WB; ELISA; IP; CoIP; ReporterAssays; Purification; Amine Reactive Labeling
    血管生长素(ANG)重组蛋白用法
    PBS或其它。
    储存
    避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。
    稳定性
    热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。
    血管生长素(ANG)重组蛋白


    Recombinant Angiogenin (ANG)

    Organism Species: Bos taurus; Bovine (Cattle)

    Instruction manual

    FOR IN VITRO USE AND RESEARCH USE ONLY

    NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

    11th Edition (Revised in May, 2016)

    血管生长素(ANG)重组蛋白[ PROPERTIES ]

    Source: Prokaryotic expression. Host: E. coli

    Residues: Ala24~His148

    Tags: N-terminal His-Tag

    Tissue Specificity: Liver. Subcellular Location: Secreted. Purity: >98%

    Traits: Freeze-dried powder

    Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300. Original Concentration: 200ug/mL

    Applications: SDS-PAGE; WB; ELISA; IP; CoIP; ReporterAssays; Purification;

    Amine Reactive Labeling. (May be suitable for use in other assays to be determined by the end user.)

    Predicted isoelectric point: 9.2

    Predicted Molecular Mass: 15.9kDa

    Accurate Molecular Mass: 18kDa as determined by SDS-PAGE reducing conditions. [ USAGE ]

    Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0

    mg/mL. Do not vortex.

    [ STORAGE AND STABILITY ]

    Storage: Avoid repeated freeze/thaw cycles. Store at 2-8

    oC for one month. Aliquot and store at -80

    oC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate

    was determined by accelerated thermal degradation test, that is, incubate the

    protein at 37

    oC for 48h, and no obvious degradation and precipitation were

    observed.The loss rate is less than 5% within the expiration date under

    appropriate storage condition. [ SEQUENCE ]

    血管生长素(ANG)重组蛋白[ IDENTIFICATION ]

    Figure 1. Gene Sequencing (Extract)

    Figure 2. SDS-PAGE



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