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左右决定因子2(LEFTY2)重组蛋白

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产品名称: 左右决定因子2(LEFTY2)重组蛋白
产品型号: LEFTY2
产品展商: 蛋白/多肽/抗原
产品文档: 无相关文档

简单介绍

左右决定因子2(LEFTY2)重组蛋白蛋白质的不同在于其氨基酸的种类、数目、排列顺序和肽链空间结构的不同,蛋白质的氨基酸序列由对应基因所编码,空间结构决定于氨基酸序列本身,通过氢键或范德华力等作用力实现。蛋白质不仅具有一级结构,还具有二级、三级结构,甚至四级结构。一级结构只是蛋白质的基本结构,称之为低级结构。左右决定因子2(LEFTY2)重组蛋白如果想让蛋白质有更多的生物功能则必须赋予它们更复杂的空间结构,称之为高级结构。应用重组DNA将目标基因进行表达得到的


左右决定因子2(LEFTY2)重组蛋白  的详细介绍

左右决定因子2(LEFTY2)重组蛋白

  • 物种Homo sapiens (Human,人)相同的名称,不同的物种。
  • 片段与标签Tyr245~Asp356 with N-terminal His Tag宿主E.coli
  • 内毒素水平<1.0EU/µg(LAL法测定)
  • 亚细胞定位分泌
  • 预测分子量13.9kDa
  • 实际分子量17kDa(差异分析请参阅说明书)
  • 缓冲液成份磷酸盐缓冲液(pH7.4,含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.)
  • 性状冻干粉
  • 纯度> 95%
  • 等电点6.0
  • 应用SDS-PAGE; WB; ELISA; IP; CoIP; ReporterAssays; Purification; Amine Reactive Labeling
    左右决定因子2(LEFTY2)重组蛋白用法
    PBS或其它。
    储存
    避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。
    稳定性
    热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。
    左右决定因子2(LEFTY2)重组蛋白


    Recombinant Left/Right Determination Factor 2 (LEFTY2)

    Organism Species: Homo sapiens (Human)

    Instruction manual

    FOR IN VITRO USE AND RESEARCH USE ONLY

    NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

    11th Edition (Revised in May, 2016)

    左右决定因子2(LEFTY2)重组蛋白[ PROPERTIES ]

    Source: Prokaryotic expression. Host: E. coli

    Residues: Tyr245~Asp356

    Tags: N-terminal His-Tag

    Tissue Specificity: Uterus. Subcellular Location: Secreted. Purity: >95%

    Traits: Freeze-dried powder

    Buffer formulation: PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01%

    sarcosyl and Proclin300. Original Concentration: 200ug/mL

    Applications: SDS-PAGE; WB; ELISA; IP; CoIP; ReporterAssays; Purification;

    Amine Reactive Labeling. (May be suitable for use in other assays to be determined by the end user.)

    Predicted isoelectric point: 6.0

    Predicted Molecular Mass: 13.9kDa

    Accurate Molecular Mass: 17kDa as determined by SDS-PAGE reducing conditions. Phenomenon explanation:

    The possible reasons that the actual band size differs from the predicted are as

    follows:

    1. Splice variants: Alternative splicing may create different sized proteins from the same gene.

    2. Relative charge: The composition of amino acids may affects the charge of the protein. 3. Post-translational modification: Phosphorylation, glycosylation, methylation etc. 4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to

    give the active form. 5. Polymerization of the target protein: Dimerization, multimerization etc. [ USAGE ]

    Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex. [ STORAGE AND STABILITY ]

    Storage: Avoid repeated freeze/thaw cycles. Store at 2-8

    oC for one month. Aliquot and store at -80

    oC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate

    was determined by accelerated thermal degradation test, that is, incubate the

    protein at 37

    oC for 48h, and no obvious degradation and precipitation were

    observed.The loss rate is less than 5% within the expiration date under

    appropriate storage condition. [ SEQUENCE ]

    左右决定因子2(LEFTY2)重组蛋白[ IDENTIFICATION ]

    Figure 1. Gene Sequencing (Extract)

    Figure 2. SDS-PAGE


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